David Duncan has served Ahmed Toure and Jackson Owusu query letters, following their failure to turn up for training sessions.
The reports indicate the duo have not trained with the side since Thursday and have been served with query letters.
They have a deadline of Friday to answer the queries, before Coach Duncan will prescribe sanctions for their actions.
Jackson Owusu, former Berekum Chelsea player and the Ivorian import, Ahmed Toure joined the Porcupine Warriors this season, but have both established themselves in the team
Asante Kotoko have been struggling in recent times having won one in their last 4 games in all competitions.
The champions have a tie against lower division side Wassaman on Wednesday in the Elite Cup in an attempt to overturn a 0-1 first leg defeat last week to the Tarkwa based side.